這是一個(gè)雙表達(dá)框載體，CMV啟動(dòng)子后有MCS供插入外源基因，hEF1α啟動(dòng)子調(diào)控Cycle 3 GFP-Zeocin™ fusion的表達(dá)，可以做到既有熒光蛋白來(lái)做轉(zhuǎn)染指示，且熒光基因表達(dá)和外源基因表達(dá)又不會(huì)互相干擾，而且Zeocin也可以用來(lái)做穩(wěn)定性細(xì)胞篩選。另外，由于EM-7啟動(dòng)子的存在，Cycle 3 GFP-Zeocin™ fusion在E.coli中也可以表達(dá)，可以用來(lái)做原核熒光定位。

Three pTracer™ mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin™ . Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin™ fusion.

All three pTracer™ vectors have the following features:

• Cycle 3 GFP-Zeocin™ fusion for selection in mammalian cell lines
• BGH polyA signal and transcription termination sequences to enhance mRNA stability

The pTracer™-SV40 vector offers:

• SV40 promoter for high-level constitutive expression of your gene of interest
• CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigen

The pTracer™-CMV2 vector offers:

• CMV promoter for high-level constitutive expression of your gene of interest
• EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
• Ampicillin resistance gene for selection in E. coli

The pTracer™-EF vectors offer:

• EF-1α promoter for high-level constitutive expression of your gene of interest
• CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
• C-terminal V5 epitope tag for simple detection of recombinant fusion proteins with an Anti-V5 Antibody
• C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His（C-term） Antibody
• Multiple cloning site supplied in three reading frames to simplify cloning in frame with the C-terminal tag